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. 2018 Jan 2;23(1):91. doi: 10.3390/molecules23010091

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of GE on MAPK pathway and cross-talk among MAPK pathways in FLS of AA rats. The proteins expression of MAPK signalling pathway were assessed by western blot on day 21 after immunization, as described in Materials and Methods. (A) Western blot for JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38, and quantitative evaluation of these proteins in FLS in vivo; (B–D) Western blot for JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38, and quantitative evaluation of these proteins in FLS in vitro with SP600125, PD98059, and SB203580 (10 μM), respectively. Values are presented as means ± SD (n = 3). ^## p < 0.01 vs. the non-immunized group; ** p < 0.01 vs. the AA model.

Effects of GE on MAPK pathway and cross-talk among MAPK pathways in FLS of AA rats. The proteins expression of MAPK signalling pathway were assessed by western blot on day 21 after immunization, as described in Materials and Methods. (A) Western blot for JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38, and quantitative evaluation of these proteins in FLS in vivo; (B–D) Western blot for JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38, and quantitative evaluation of these proteins in FLS in vitro with SP600125, PD98059, and SB203580 (10 μM), respectively. Values are presented as means ± SD (n = 3). ^## p < 0.01 vs. the non-immunized group; ** p < 0.01 vs. the AA model.