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. 2018 Feb 7;23(2):350. doi: 10.3390/molecules23020350

Figure 5.

Figure 5

(a) Time course for the reaction of native or derivatized Hb (Hb + GL, Hb + BSA + GL, Hb + BSA + DSS, Hb + BSA + DSS + GL, Hb + Rbr + GL cf. Materials and Methods) with nitrite. Conditions: 25µM protein, 200µM nitrite, PBS 7.4, room temperature; (b) Time course for the reaction of Hb from different sources (cow, goat, horse, sheep) with nitrite. Conditions: 30 µM protein, 250 µM nitrite, PBS 7.4, room temperature; (c) Plots of k1 and k2 vs. NO2 concentration for the reaction of different derivatized oxiHb with NO2 at pH 7.4, aerobic; Left panel: up-polyHb, down- copolyHbBSA GL, right: up-copolyHbRbr, down-copolyHbBSA DSS. See also Supplementary Information Tables S1, S3 and S6 and Figures S17–S24.