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Surface plot; (A) Reaction temperature and amount of enzyme (X2X3) against yield (substrate ratio 5:1; reaction time 15 h); (B) Reaction time and amount of enzyme (X2X4) against yield (substrate ratio 5:1; temperature 42 °C); and (C) Reaction time and reaction temperature (X3X4) against yield (substrate ratio 5:1; amount of enzyme 1.83%, w/v).
