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Coded and actual levels of variables for response surface methodology (RSM) experimental design.
| Variable | Symbol | Coded Level | ||||
|---|---|---|---|---|---|---|
| −2 | −1 | 0 | 1 | 2 | ||
| Corresponding Operation Value | ||||||
| PA:KA (mmol) | X1 | 1 | 3 | 5 | 7 | 9 |
| Enzyme loading (%, w/v) | X2 | 0.33 | 1.11 | 1.83 | 2.60 | 3.33 |
| Temperature (°C) | X3 | 34.0 | 38.0 | 42.0 | 46.0 | 50.0 |
| Reaction time (h) | X4 | 5 | 10 | 15 | 20 | 25 |
