Figure 1.

Effect of base of nucleotide at the loop of G-quadruplex RNA on the binding of heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1). Electrophoretic mobility shift assay (EMSA) was performed with hnRNPA1 and ³²P-labeled RNAs using 8% PAGE in 1 × TBE buffer with 20 mM NaCl in 4 °C for 2 h (80 V). The structures of DNA loop (DNA-loop-Gq), abasic RNA loop (D-abasic-loop-Gq), abasic DNA loop (R-abasic-loop-Gq) and 2’-O-methylribonucleotide loop (2′-O-Me-loop-Gq) are indicated at upper.