Figure 4.

β-Escin reduced capillary formation in vivo. (A) Effect of β-escin on angiogenesis in CAM assay. The CAM models were prepared using 7-day-old chick embryos treated as described in Materials and methods. Sterilized silicone ring was positioned on CAM surface and 30 µL of the sample under test was placed within the ring. After incubation for 72 h, CAMs were photographed with a digital camera. Each group contained five CAMs and the experiment was repeated three times; (B) The results are summarized in the graph as the angiogenesis index (the mean ± SD of new vessel branch points per field) for each experimental variable. Error bars represent ± SD (*** p < 0.001 versus bFGF alone).