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. 2018 May 16;53(2):620–632. doi: 10.3892/ijo.2018.4408

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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TRIM27 is upregulated in CRC cell lines and can be regulated by siRNA and plasmid transfection. (A) mRNA expression of TRIM27 in different CRC cell lines compared with NCM460 normal colon epithelial cells. (B) Relative protein levels of TRIM27 in different CRC cell lines compared with NCM460 normal colon epithelial cells. (C) Relative mRNA expression of TRIM27 in LoVo cells following siRNA transfection. (D) Relative protein levels of TRIM27 in LoVo cells following siRNA transfection. (E) Relative mRNA expression of TRIM27 in HCT116 cells following plasmid transfection. (F) Relative protein levels of TRIM27 in HCT116 cells following plasmid transfection. Data are presented as the mean ± standard deviation from three independent experiments; ^*P<0.05, ^**P<0.01 and ^***P<0.001, compared with controls. TRIM27, tripartite motif-containing 27; CRC, colorectal cancer; NC, negative control sequence; siRNA, small interference RNA.