Figure 7.

TRIM27 promotes the activation of p-AKT and the epiethlium-mesenchymal transition process in colorectal cancer cells. (A) Expression levels of p-AKT, AKT, E-cadherin, N-cadherin and vimentin in LoVo and HCT116 cells following TRIM27 knockdown and overexpression were detected using western blot analysis. (B) Relative protein levels of p-AKT, E-cadherin, N-cadherin and vimentin were quantified in LoVo and HCT116 cells. Data are presented as the mean ± standard deviation from three independent experiments; ^**P<0.01 and ^***P<0.001, compared with controls. TRIM27, tripartite motif-containing 27; si-TRIM27, small interference RNA target TRIM27; p-, phosphorylated; NC, negative control sequence.