Figure 5.

Time-response effects of diosmetin on cell cycle regulatory molecules. (A) LNCaP and PC-3 cells; both asynchronous cells were exposed to increasing times (0, 6, 12 and 24 h) of diosmetin (20 µM). Total cell lysates were prepared and using SDS-PAGE, lysates proteins were resolved and then subjected to western blot analysis for the evaluation of cyclin D1, cyclin E, Cdk2, Cdk4 and p27^Kip1. Lanes marked ‘0’ are the DMSO control (0.2%)-treated cells. For protein band density, densitometric analysis was performed and protein expression levels were correlated to express relative controls (B and C). For loading control blots were stripped and reprobed with GAPDH. ^*P<0.05 for time points vs. vehicle controls.