Figure 7.

Dose-response effects of diosmetin on signaling pathways in human prostate cancer cells. (A) LNCaP and PC-3 cells were exposed to two concentrations of diosmetin (10 and 20 µM) for 24 h. Total cell lysates were prepared and using SDS-PAGE, lysates proteins were resolved and then subjected to western blot analysis for the evaluation of c-Myc, Cdk4, cyclin D1, FOXO3a and p27^Kip1. Lanes marked ‘0’ are the DMSO control (0.2%)-treated cells. For protein band density, densitometric analysis was performed and protein expression levels were correlated to express relative controls (B and C). For loading control blots were stripped and reprobed with GAPDH. ^*P<0.05 for diosmetin concentration vs. vehicle controls.