Table 1.
LC-MS * and RP-HPLC analysis and summary.
| Phytochemicals Identified in H. verticillata by LC-MS | Empirical Formula | Mono-isotopic Mass (Da) 1 | Confirmation by RP-HPLC Standard |
|---|---|---|---|
| Cadina-10(15)-en-3-one | C15H22O | 218.167 | |
| Aromadendr-1(10)-en-9-one | C15H22O | 218.167 | |
| 7,11,15-Trimethyl-3-methylenehexadecane -1,2-diol | C20H40O2 | 312.303 | |
| Rosmarinic acid | C18H16O8 | 360.315 | |
| Deoxydehydropodophyllotoxin 2 | C22H18O7 | 394.105 | (+) |
| Dehydrodesoxypodophyllotoxin | C22H18O7 | 394.105 | |
| ß-Apopicropodophyllin | C22H20O7 | 396.121 | |
| Hyptinin | C22H20O7 | 396.121 | |
| Isodeoxypodophyllotoxin | C22H22O7 | 398.136 | |
| Deoxypicropodophyllin | C22H22O7 | 398.136 | |
| 4-Demethylpodophyllotoxin | C21H20O8 | 400.116 | (+) |
| (-)-Yatein | C21H20O8 | 400.152 | (+) |
| Dehydropodophyllotoxin 2 | C22H18O8 | 410.100 | (+) |
| Podophyllotoxin | C22H22O8 | 414.131 | (+) |
| ß-Peltatin | C22H22O8 | 414.131 | |
| Oleanolic acid 3 | C30H48O3 | 456.360 | (+) |
1 ChemSpider—Royal Society of Chemistry; 2 Lignans in the five lignan mix supplied by UWI Dept. of Chemistry; (+) tested and confirmed to be present using HPLC analysis with the relevant standard; Bold—five phytochemicals identified through both LC-MS and RP-HPLC analysis; 3 Phytochemical identified through RP-HPLC alone; * Exact mass measurements using a High Resolution instrument (Waters LCT ToF MS, with a Waters Alliance 2690 HPLC and Mass Lynx V4.1 software).