Figure 3.

PSM specifically induces colocalization of aggregated mitochondria and CYTO-ID puncta. (A and C) Normally grown and (B) overgrown A375 cells were treated with (A and B) PSM (100%) or (C) 100 ng/ml TRAIL for 24 h at 37°C, and were stained with CYTO-ID and MitoTracker Red CMXRos. In the panels labeled 'Overlay', the images in the white boxes with solid lines represent the expansion of images in the smaller boxes with dotted lines. Images were obtained and analyzed using an EVOS FL Cell Imaging system. Scale bar, 100 µm. PSM, cold plasma-stimulated medium.