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. 2018 May 21;53(2):503–514. doi: 10.3892/ijo.2018.4413

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Copyright: © Ito et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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PSM induces ACD during the initial 24 h post-stimulation. (A) HOS, (B) SAOS-2 and (C) MG63 cells were treated with PSM (25 and 100%), 5 mM 3-MA and 100 nM Baf alone or in combination for 24 h, and cell viability was analyzed using WST-8 assay in triplicate. Data are presented as the means ± standard deviation of a representative experiment (n=3). Data were analyzed by one-way analysis of variance followed by Tukey's post hoc test. ^###P<0.001 vs. control; NS, not significant vs. control; ^*P<0.05; ^***P<0.001. 3-MA, 3-methyladenine; Baf, bafilomycin A; PSM, cold plasma-stimulated medium.