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. 2018 Jun 13;53(2):539–550. doi: 10.3892/ijo.2018.4439

Figure 4.

Figure 4

Microwave (MW) hyperthermia triggers cell death through caspase-3-mediated apoptosis. H460, PC-9 and H1975 cells were treated with or without Ac-DEVD-CHO for 3 h prior to MW hyperthermia (43°C for 90 min), then allowed to recover at 37°C until 24 h. (A) Cell viability was determined by CCK-8 assay. Data are expressed as the means ± SEM of 3 independent experiments. *P<0.05 vs. the control group, **P<0.01 vs. the control group. (B) Quantitative analyses of Annexin-FITC positive H460, PC-9 and H1975 cells are shown. *P<0.05 vs. the control group, **P<0.01 vs. the control group. (C) Caspase-3 activity following treatment was analyzed. Data are expressed as the means ± SEM of 3 independent experiments. **P<0.01 vs. the control group, ***P<0.001 vs. the control group; #P<0.05 vs. MW treatment group, ###P<0.001 vs. MW treatment group.