Figure 5.

Microwave (MW) hyperthermia treatment increases reactive oxygen species (ROS) levels. Cells were seeded onto 6-well plate at 5×10⁵/well and then exposed to the water bath or MW radiation at 43°C for 90 min. Following incubation for 6 h, cells were pre-loaded with DCFH-DA and then mounted under a fluorescence microscope. (A-C) Representative photographs from DCF fluorescence staining in the H460, PC-9 and H1975 cells, respectively. Scale bars, 200 µm. (D) Quantitative analyses of the mean fluorescence intensity. ^*P<0.05 vs. the control group in H460 cells, ^***P<0.001 vs. the control group in PC-9 cells, ^**P<0.01 vs. the control group in H1975 cells.