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. 2018 Feb 22;23(2):477. doi: 10.3390/molecules23020477

Figure 3.

Figure 3

Comparative effects of TQ-1-TQ-3 on collagen induced cytotoxicity, ATP release, relative [Ca2+]i mobilization and on surface FITC-P-selectin expression in human platelets. Washed human platelets (3.6 × 108 cells/mL) were pre-incubated with TQ-1, TQ-2 (250 μM) and TQ-3 (5 μM) or a solvent control (0.5% DMSO) to check the cytotoxicity (A) and subsequently treated with 1 μg/mL of collagen to stimulate ATP release reaction (B); to induce the cytoplasmic influx of Ca2+ from intracellular stores (C); or to check the direct binding of FITC-P-selectin (D) as described in the materials and methods section. Data are presented as the means ± S.E.M. (n = 4). ** p < 0.01 and *** p < 0.001 compared with the DMSO group. # p < 0.05 and ### p < 0.001 compared with the collagen induced group.