Figure 4.
Effects of ruthenium complexes on the phosphorylation of Fyn/Lyn-Syk and MAPKs induced by collagen in human platelets. Washed platelets (1.2 × 109 cells/ml) were incubated with solvent control (0.5% DMSO) or 250 μM TQ-1 and TQ-2 and 5 μM TQ-3 and then treated with 1 μg/mL collagen to induce platelet activation. The subcellular extracts were analyzed for the phosphorylation of Fyn (A); Lyn (B); Syk (C); Akt (D); JNK1(E) and p38 MAPK (F) by western blotting. Data are presented as the mean ± SEM (n = 4). * p < 0.01 and *** p < 0.001 compared with the DMSO group, # p < 0.05, ## p < 0.01 and ### p < 0.001 compared with the collagen induced group.