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. 2018 Mar 8;23(3):615. doi: 10.3390/molecules23030615

Figure 6.

Figure 6

Inhibition of NF-κB activation of RANKL induced osteoclast in RAW 264.7 cells by AM extracts. (a,b) AM extracts altered the phosphorylation of the IκBα and inhibited nuclear translocation of NF-κB. (c–f) Band intensities of pIκBα/IκBα and NF-κB for AM water and ethanol extracts. Cells were exposed to RANKL (50 ng/mL) in the presence and absence of AM extracts for 3 days. The expression of the proteins were determined by Western blot analysis and Beta actin was used as loading control. RANKL treated group was considered as 100% for densitometric analysis. All data are presented as the mean ± SD of three independent experiments performed with n = 3. *** p < 0.001 vs. RANKL treated group; and # p < 0.05, ## p < 0.01, ### p < 0.001 vs. Con group. Con; positive control, (which was not treated), RANKL; negative control, (which was treated with only RANKL).