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. 2018 Mar 8;23(3):615. doi: 10.3390/molecules23030615

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Inhibitory effects of AM extracts on c-Fos and NFATc1 expression in RAW 264.7 cells induced by RANKL. (a,b) AM extracts inhibited the expression of c-Fos and NFATc1; (c–f) Band intensities for c-Fos and NFATc1as percentages of the RANKL treated group (set as 100%), respectively for AM water and ethanol extracts. RANKL treated group was considered as 100% for densitometric analysis. Cells were exposed to RANKL (50 ng/mL) in the presence and absence of AM extracts for 3 days. The expression of the proteins were determined by Western blot analysis and Beta actin was used as loading control. All data are presented as the mean ± SD of three independent experiments performed with n = 3. *** p < 0.001 vs. RANKL treated group; and ^## p < 0.01, ^### p < 0.001 vs. Con group. Con; positive control, (which was not treated), RANKL; negative control, (which was treated with only RANKL).