Figure 8.

Inhibitory effects on gene expression of Cathepsin K, TRAP, Calcitonin receptor, Integrin β₃ during RANKL induced osteoclast in RAW 264.7 cells by AM extracts. (a,b) AM extracts inhibited the expression of Cathepsin K, TRAP, Calcitonin receptor, Integrin β₃; (c–j) Band intensities for Cathepsin K, TRAP, Calcitonin receptor, Integrin β₃ as percentages of the RANKL treated group (set as 100%), respectively. RANKL treated group was considered as 100% for densitometric analysis. Cells were exposed to RANKL (50 ng/mL) in the presence and absence of AM extracts for 3 days. The protein expression was determined by Western blot analysis and Beta actin was used as loading control. All data are presented as the mean ± SD of three independent experiments performed with n = 3. ** p < 0.01, *** p < 0.001 vs. RANKL treated group; and ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 vs. Con group. Con; positive control, (which was not treated), RANKL; negative control, (which was treated with only RANKL).