Figure 2.

CH-5 inhibited cell migration and invasion in HGC-27 cells. (A) The effect of CH-5 on HGC-27 cell migration was evaluated by a wound healing assay. HGC-27 cells were scratched and treated with 0, 10, 20, and 40 μM of CH-5 for 0 and 24 h. The migration was observed under a phase-contrast microscope at a magnification of 40×. Migration inhibition (%) after treatment with CH-5 was calculated, and quantitative results are illustrated in the right panel; (B) The inhibitory effect of CH-5 on HGC-27 cell migration was detected by a Transwell assay. Cells in serum-free medium were plated onto the upper chamber of the Transwell. Complete medium (10% serum) containing CH-5 at the indicated doses was added to the lower chamber. After 24 h, cells on the bottom side of the Transwell membrane were stained and observed by manual counting and measuring absorbance at 490 nm. Migration Inhibition (%) was calculated and quantitative results are illustrated in the right panel; (C) For the invasion assay, the Transwell membrane was pre-coated with Matrigel, following which the cells were plated and treated as described above. Invasion Inhibition (%) was calculated and quantitative results are illustrated in the right panel. In all experiments, the data represent the mean ± SD of three experiments. * p ≤ 0.05, ** p ≤ 0.01, and *** p ≤ 0.001 vs. DMSO group.