Table 6.

Analytical conditions of HPLC system for analyzing two markers.

Parameters	Conditions
Column	Zorbax extended-C18 (C18, 4.6 mm × 150 mm, 5 µm)
Flow rate	0.8 mL/min
Injection volumn	10 μL
UV detection	330 nm
Run time	30 min
Gradient	Time (min)	% A	% B
	0	10	90
	7	10	90
	8	20	80
	20	25	75
	21	100	0
	25	10	90
	30	10	90