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. 2018 Feb 3;23(2):324. doi: 10.3390/molecules23020324

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Determination of optimal reaction conditions for Mtb DdlA activity assay. (A–F) represented the effect of alanine concentration, DdlA concentration, ATP concentration, pH, incubation time, and temperature on the enzyme activity respectively; (G) The effect of alanine concentration on Tb-DdlA activity analyzed by the double reciprocal plot; (H) The effect of ATP concentration on Tb-DdlA activity analyzed by the double reciprocal plot.