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Effect of site marker probes on HSA-SSD and HSA-PF binding at 20 °C. (A,B) stand for the competition of War/Ibu with 1–18 nM SSD/PF at CHSA = 1 μM, CWar = 10 nM, CIbu = 184 nM; (C,D) are for the competition of SSD/PF with 1-18 nM War/Ibu at CHSA = 1 μM, CSSD = 3 μM, CPF = 3 μM; [Q], CSSD or CPF or CSSD or CSSD = 0, 1, 3, 6, 9, 12 and 18 nM, respectively. War, Warfarin; Ibu, Ibuprofen; SSD, saikosaponin D; PF, paeoniflorin.
