Table 1.
The IC50 values of acteoside, forsythoside B and poliumoside in various antioxidants assays.
| Assays | Acteoside μM |
Forsythoside B μM |
Poliumoside μM |
Trolox μM |
|---|---|---|---|---|
| FRAP | 5.4 ± 0.6 a | 7.7 ± 0.2 c | 8.1 ± 0.2 d | 6.8 ± 0.4 b |
| CUPRAC | 4.8 ± 0.4 a | 6.1 ± 0.5 b | 6.5 ± 0.3 c | 7.8 ± 0.2 d |
| PTIO•-scavenging (pH 4.5) | 247.3 ± 21.2 b,B | 352.2 ± 20.8 c,B | 219.5 ± 10.4 b,B | 164.0 ± 7.5 a |
| PTIO•-scavenging (pH 7.4) | 120.8 ± 2.5 a,A | 125.5 ± 6.5 b,A | 127.2 ± 2.1 c,A | 223.7 ± 6.5 d |
| ABTS+•-scavenging | 12.5 ± 1.9 a | 14.0 ± 2.6 b | 19.9 ± 1.7 c | 25.8 ± 4.8 d |
| DPPH•-scavenging | 7.6 ± 0.1 a | 8.7 ± 0.1 b | 10.9 ± 0.6 c | 24.2 ± 0.3 d |
| •O2−-scavenging | 731.0 ± 1.7 b | 262.6 ± 3.3 a | 266.3 ± 4.6 a | 1205.2 ± 19.8 c |
The IC50 value (in μg/mL unit) was defined as the final concentration of 50% radical inhibition or relative reducing power and was calculated by linear regression analysis and expressed as the mean ± SD (n = 3). The linear regression was analyzed using Origin 6.0 professional software. The IC50 value (in μg/mL unit) was conversed into that of an μM unit and collected in brackets. The IC50 value in the μM unit with different superscripts (a, b, c, or d) in the same row are significantly different (p < 0.05); The IC50 value in the μM unit in PTIO assay with different superscripts (A or B) are significantly different (p < 0.05) between at pH 4.5 and pH 7.4. Trolox is the positive control. The dose-response curves are listed in Suppl. 1.