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Translation is initiated at the uORF AUG codon. HeLa cells were transiently transfected with LUC constructs containing either (1) LUC downstream of a CMV promoter or (2) a construct containing CHOP-uORF/LUC in-frame fusion downstream of the CMV promoter. Seventy-two hours after transfection, whole cell lysates were prepared and analyzed for the luciferase protein content by western blotting as described in Materials and Methods.
