Figure 6.

PRE experiments with SBD(∆CDE)-y with spin labeling at residue 537. (a) The spin-labeling position in the solution structure of SBD(∆CDE)-y is shown, in which, S537 was exchanged to cysteine to facilitate covalent labeling with MTSL; (b) Representative spectral changes caused by the paramagnetic effect. In the left two panels, 2D ¹H-¹⁵N HSQC spectra are overlaid for the paramagnetic spin labeled sample (red) and for the sample with a diamagnetic label (black) in the absence of the NR-peptide. In the two panels on the right, the corresponding spectral comparison in the presence of 5.0 mM NR-peptide. The concentration of SBD(∆CDE)-y was adjusted to 0.5 mM.