Table 4.
Optimum conditions derived by response surface methodology (RSM) and validation set for synthesis of dilaurylazelate ester.
| Enzyme Amount (gram) | Independent Variables | Conversion% | |||||
|---|---|---|---|---|---|---|---|
| Reaction Time (min) | Reaction Temperature (°C) | Molar Ratio of Substrates, AA:LA (mole) | Actual Value | Predicted Value | RSE (%) | ||
| Validation Set | 0.20 | 180 | 50 | 1:6 | 84.23 | 83.01 | 1.47 |
| 0.30 | 300 | 52 | 1:5 | 91.19 | 89.05 | 2.40 | |
| 0.20 | 360 | 52 | 1:6.5 | 90.99 | 89.84 | 1.28 | |
| 0.16 | 250 | 55 | 1:5.5 | 87.17 | 87.75 | 0.66 | |
| 0.10 | 360 | 52 | 1:6 | 95.10 | 93.54 | 1.66 | |
| Optimal conditions | 0.14 | 360 | 46 | 1:4.1 | 95.38 | 96.23 | 0.88 |