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. 2018 Apr 10;23(4):861. doi: 10.3390/molecules23040861

Figure 2.

Figure 2

Cloning, amino acid sequence alignment, and an unrooted phylogenetic tree of DFR obtained from deduced amino acid sequences of 13 DFR homologs. (a) a DFR cDNA fragment was amplified from V. bellula leaf tissue by RT-PCR. M: DNA marker, 1: DFR cDNA fragment, CT: negative control; (b) an unrooted phylogenetic tree was built from amino acid sequences of 13 DFR homologs; (c) amino acid sequence alignment were develop from 13 DFR homologs; “*”: the same amino acid in all sequences; “:”: conserved amino acid residues; “.”: half conserved amino acid residues; “######”: potential NADPH/NADH binding domain; amino acids underlined form a potential substrate specificity domain of DFR. AtDFR: Arabidopsis thaliana DFR; CmDFR: Crataegus monogyna DFR; GmDFR1: Glycine max DFR1; GmDFR2: Glycine max DFR2; LjDFR: Lotus japonicas DFR; MdDFR: Malus domestica DFR; MtDFR: Medicago truncatula DFR; NgDFR: Nekemias grossedentata DFR; PaDFR: Prunus avium DFR; PpDFR: Pyrus pyrifolia DFR; PlDFR: Paeonia lactiflora DFR; VvDFR: Vitis vinifera DFR.