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. 2018 Apr 10;23(4):861. doi: 10.3390/molecules23040861

Figure 5.

Figure 5

Increase of anthocyanins in transgenic tobacco flowers overexpressing VbDFR. (a) cassettes of pBI121 and pBI121-VbDFR vectors show VbDFR controlled by 35S promoter for overexpression, and red pigmentation was enhanced in flowers of two VbDFR transgenic tobacco lines (L1 and L2) compared with vector control flower; (b) images show fragments amplified by genomic DNA-based PCR and RT-PCR; (c) absorbance values for anthocyanin extracts were significantly higher from flowers of VbDFR transgenic tobacco lines (L1 and L2) than from vector control flowers; (d) TLC profiles show anthocyanidins from the butanol:HCl hydrolysis of anthocyanins extracts from transgenic tobacco line (L1) and vector control plants; (e) HPLC profiles show anthocyanidins from butanol:HCl hydrolysis of anthocyanins extracted from transgenic tobacco line (e-1) and vector control (e-2) plants. e-3: authorized standards, Del: delphinidin, Cy: cyanidin, Pel: pelargonidin. * significant difference between transgenic and wild-type control flowers.