Figure 3.

Proliferation of draining lymph node cells (A,C) and splenocytes (B,D) from control vehicle-treated rats, and rats treated with cyclic myelin basic protein (MBP) peptide analogue, cyclo(87–99)[Arg⁹¹, Ala⁹⁶]MBP_87–99, isolated 10 days post-immunization with MBP_72–85 for the induction of experimental autoimmune encephalomyelitis (EAE). Cells were stimulated ex vivo with increasing concentrations of linear MBP_72–85 agonist (A,B) or cyclo(87–99)[Arg⁹¹, Ala⁹⁶]MBP_87–99 peptide (C,D) (n = 4 per group). Data are from one (A,B), or one representative of two (C,D) independent experiments. Statistical significance after pair-wise comparisons (using Student’s t-test) of each experimental group with the control untreated, MBP_72–85-induced EAE group is shown (*, p < 0.05).