Table 1.

Primers used in SjFMRFamide cDNA identification and full-length amplification.

Primer Name	Sequence (5′–3′)	Position
FMRF-F	CAGCGTGWMCCTTYKTATCTT	172–192
FMRF-R	CAAAWAAGTCAATCWCGCAGG	1621–1641
5′-FMRF-outer	GATGACTTAGGTCCGACTAC	252–271
5′-FMRF-inner	GATGGAGCCGCACACACAGAC	193–213
3′-FMRF-outer	GCAAAGGGTGTCTGGAAGGT	1307–1326
3′-FMRF-inner	GACACACACGCAAACATACAC	1469–1489
RT-actin-F	TGAGAGGGAGATTGTGCGTG	815–834
RT-actin-R	GAACATAGATTCTGGAGCACGG	968–989
RT-FMRF-F	CGTCATCGCCATCTACTGTC	366–385
RT-FMRF-R	CGCTTGCTTCTCAGTCCATC	514–533
FMRF-probeF	CCCAAGCGTGATGCGTTGTTGGAGT	330–348
FMRF-probeR	CCGGAAGCGCTTGCTTCTCAGTCCATC	514–533