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. 2018 Jan 22;23(1):236. doi: 10.3390/molecules23010236

Figure 1.

Figure 1

Auto- and transphosphorylation of recombinant Brassica oleracea LRR-RLK (BoLRR-RLK) proteins expressed in Escherichia coli. Antiphosphothreonine and antiphosphotyrosine immunoblots showing patterns of auto- and transphosphorylation. Not all receptor kinases were capable of transphosphorylating proteins following induction in E. coli. Auto- and transphosphorylation activity of recombinant and E. coli proteins was measured after receptor kinases had been expressed for 16 h. Each sample shows total crude proteins, including FLAG-tagged receptor kinase, extracted from E. coli and subjected to 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) before transfer to polyvinylidene difluoride (PVDF) membranes. Membranes were immunoblotted (IB) with the antibodies indicated. The asterisks (*) indicate which leucine-rich repeat receptor-like kinases (LRR-RLKs) shown autophosphorylation or auto-and transphosphorylation.