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Analysis of auto- and transphosphorylation activity of 43 receptor kinases. All kinases were expressed as recombinant fusion proteins with an N-terminal Flag tag. Not all LRR-RLK proteins had cytoplasmic domains (CDs) capable of autophosphorylation activity in E. coli; only LRR-RLKs with autophosphorylation activity showed transphosphorylation kinase activity. P: phosphorylation.
