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Cell fractionation and location of cellulase activity in wild-type B. licheniformis and recombinant cell.
| Cell Fractionation | Wild Type | Recombinant (INP-Cellulase) | ||
|---|---|---|---|---|
| (mU/mL) | (%) 1 | mU/mL | (%) 1 | |
| Cell Culture Medium (Supernatant) | 17.2 | 14.8 | 468.0 | 47.5 |
| Periplasm | 26.3 | 22.7 | 307.4 | 31.2 |
| Cytoplasmic (Intracellular) | 71.0 | 61.3 | 18.7 | 1.9 |
| Membrane | 1.4 | 1.2 | 191.1 | 19.4 |
| Total Activity | 115.9 | 100 | 985.2 | 100 |
1 (%) indicates the fractional cellulase activity per total cellulase activity.
