Figure 6. Microscale thermophoresis measurement of dissociation constant between ^RerCpa and ^Mtb20S CP.

(A) Binding curves of ^RerCpa vs. closed- (red) and open-gate (black) ^MtbCP in presence of ATP. The gray triangles represent a dataset recorded for Cpa and open-gate proteasome in absence of nucleotide. (B) Dissociation constants and Hill coefficients for the two measured datasets.