Table 4.

Primer sequences for eleven reference genes used in the real-time qRT-PCR analysis.

Genes Name	Accession ID	Genes Description	Primer Sequence (5′→3′) Forward and Reverse	Tm/°C	Amplicon Length/bp	Amplification Efficiency	R2
18S rRNA	NC003071.7	18S ribosomal RNA	F:GATAGGAAGAGCCGACAT R:ATACGAACCGTGAAAGCG	58.5	156	87.1%	0.999
SAMDC	HO177535	S-adenosylmethionine decarboxylase	F:TAGCCTGTTCATCTACTC R:AAGAATCCTTGGAATGGT	55	82	92.2%	0.982
GAPDH	GR518033	Glyceraldehyde-3-phosphate dehydrogenase	F:TCTGACCGTTAGACTTGAGAAGG R:CTTGAGCTTACCCTCAGACTCCT	59	135	100.8%	0.991
TBP-1	GO924783	Proteasome regulatory subunit 6A homolog	F:TGCTTAGTTCCCCTAAGATAGTGA R:CTGAGACCAAACACGATTTCA	60	112	104.2%	0.993
eIF4A	GO924770	Eukaryotic initiation factor4 alpha	F:AACTCAACTTGAAGTGTTGGAGTG R:AGATCTGGTCCTGGAAAGAATATG	59	168	95.4%	0.991
E2	GO924794	Ubiquitin-conjugatingenzyme	F:CGGTTCTGTGCCAAAATGT R:CAGCTATCTCCAACGGTTCA	61.6	111	92.9%	0.990
HIS3	KM454863.1	Imidazoleglycerol-phosphate dehydratase	F:ACTTCAAGACTGATCTGCGTTTCC R:CATGGATGGCACAAAGGTTGG	63.3	80	108%	0.994
Unigene71		unknown	F:CTTGGAGGAAGAGGGACGAG R:GAGACAGGAGAGAGTGGCGG	61.3	134	93.4%	0.992
Unigene77		unknown	F:CAGGTAAAAGGAACAAACAT R:TCAATTCAGTAATCAAAGGC	56.9	98	89.6%	0.980
Unigene755		unknown	F:AAATGATAAACTGACCAAAC R:CCACTCAAGAGCCAAAATAG	55	101	91.3%	0.980
Unigene14912		unknown	F:TACACCCATGAGAATGCCAC R:TGTTACCTTGAGGAGTCGGA	59.4	110	91.2%	0.982
P5CS1	JX470539	Delta-1-pyrroline-5-carboxylate	F:ATAACCAATGCTATCCCTGAC R:TCTTAGTCGTTGCCTTGA	51	160
Cyt-Cu/Zn SOD	JQ269677	Cytosolic Cu/Zn superoxide	F:GGCTGAGTATCCCATTT R:CTGCCTTTGCTGTTCT	57	87