Table 2.
Chromatographic data, retention factor of first eluted enantiomer (k1), separation factor (α), resolution (RS), and elution order, for the chiral xanthonic analytes on ChirobioticTM R column, under multimodal chromatographic conditions.
| Analyte | Elution Mode | Mobile Phase | k1 | α | RS | First Eluted Enantiomer |
|---|---|---|---|---|---|---|
| 1 | POM | 100% MeOH | 0.27 | 1.72 | 1.23 | (S) |
| PIM | MeOH:AcOH:TEA (100:0.1:1 v/v/v) | 0.50 | 1.60 | 1.41 | (S) | |
| 2 | POM | 100% MeOH | 0.27 | 1.44 | 0.45 | (R) |
| PIM | MeOH:AcOH:TEA (100:0.01:0.01 v/v/v) | 0.48 | 1.32 | 0.81 | (R) | |
| 4 | POM | 100% MeOH | 0.23 | 1.88 | 1.00 | (S) |
| PIM | MeOH:AcOH:TEA (100:0.1:1 v/v/v) | 0.48 | 1.52 | 1.37 | (S) | |
| 5 | POM | 100% MeOH | 0.28 | 1.80 | 1.02 | (R) |
| PIM | MeOH:AcOH:TEA (100:0.1:1 v/v/v) | 0.39 | 1.65 | 1.52 | (R) | |
| 9 | NPM | Hex:EtOH (80:20 v/v) | 9.32 | 1.46 | 1.99 | (R) |
| NPM | Hex:2-PrOH (70:30 v/v) | 7.59 | 1.88 | 1.49 | (R) | |
| POM | 100% 2-PrOH | 1.44 | 1.85 | 1.32 | (R) | |
| PIM | MeOH:AcOH:TEA (100:0.1:0.1 v/v/v) | 0.22 | 1.72 | 0.87 | (R) | |
| 10 * | NPM | Hex:EtOH (90:10 v/v) | 15.78 | 1.24 | 1.77 | (R) |
| NPM | Hex:2-PrOH (80:20 v/v) | 8.41 | 1.69 | 1.73 | (R) | |
| 11 | NPM | Hex:EtOH (90:10 v/v) | 10.83 | 1.16 | 1.68 | (R) |
| 12 | NPM | Hex:EtOH (90:10 v/v) | 28.07 | 1.16 | 1.37 | (R) |
| PIM | MeOH:AcOH:TEA (100:0.1:1 v/v/v) | 0.67 | 1.26 | 0.84 | (R) | |
| 13 * | PIM | MeOH:AcOH:TEA (100:0.01:0.01 v/v/v) | 0.44 | 1.47 | 1.15 | (S) |
| 17 | NPM | Hex:EtOH (90:10 v/v) | 2.32 | 2.91 | 3.95 | (S) |
| NPM | Hex:2-PrOH (70:30 v/v) | 0.39 | 4.65 | 2.05 | (S) | |
| POM | 100% 2-PrOH | 0.29 | 2.05 | 0.91 | (S) | |
| RPM | MeOH:TEAA pH 4.2 (50:50 v/v) | 1.09 | 1.35 | 0.70 | (S) | |
| 19 | RPM | MeOH:TEAA pH 4.2 (30:70 v/v) | 1.90 | 1.21 | 1.00 | (R) |
| 20 | NPM | Hex:EtOH (90:10 v/v) | 20.33 | 1.11 | 1.18 | (R) |
| NPM | Hex:2-PrOH (50:50 v/v) | 2.13 | 1.28 | 0.91 | (R) | |
| PIM | MeOH:AcOH:TEA (100:0.1:1 v/v/v) | 1.10 | 1.11 | 0.74 | (R) | |
| 22 | NPM | Hex:EtOH (90:10 v/v) | 9.69 | 1.58 | 2.81 | (R) |
| NPM | Hex:2-PrOH (70:30 v/v) | 2.06 | 2.21 | 1.83 | (R) | |
| POM | 100% 2-PrOH | 0.61 | 1.97 | 1.00 | (R) | |
| 23 * | NPM | Hex:2-PrOH (80:20 v/v) | 2.42 | 1.52 | 1.12 | (R) |
| POM | 100% 2-PrOH | 0.41 | 1.36 | 0.50 | (R) | |
| RPM | MeOH:TEAA pH 4.2 (40:60 v/v) | 0.64 | 1.70 | 1.51 | (S) | |
| 27 | RPM | MeOH:TEAA pH 4.2 (30:70 v/v) | 15.54 | 1.09 | 0.70 | (S) |
| 28 | NPM | Hex:EtOH (90:10 v/v) | 6.41 | 1.11 | 1.13 | (S) |
| RPM | MeOH:TEAA pH 4.2 (30:70 v/v) | 15.45 | 1.25 | 1.12 | (S) | |
| 29 | RPM | MeOH:TEAA pH 4.2 (40:60 v/v) | 13.04 | 1.16 | 1.18 | (S) |
Chromatographic conditions: flow rate: 0.2 mL/min, UV detection at 254 nm; * Elution order changed with the mobile phase.