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The design of primers in ARMS-qPCR. A set of primers in ARMS is composed of 3 primers, including one common revers primer (blue) and two allele-specific (AS) forward primers (orange and green). The last base of AS primers are set purposively to be consistent with altered bases, while the two nucleotides immediately 5’ to the mutated site (marked as red) are also changed to increase the resolving power of ARMS.
