Table 1.
Comparison of heteroplasmy detection methods.
| Methods | Detection Limit | Variations Type 2 | |
|---|---|---|---|
| SNPs | Indels | ||
| Sanger sequencing | ~15% | Y | Y |
| Polymorphism ratio sequencing | ~5% | Y | Y |
| ARMS-qPCR | ~0.1% | Y (recorded) | N |
| Single-molecule PCR | ~7% [53] | Y | Y |
| Digital PCR | ~0.01% 1 | Y (recorded) | Y (recorded) |
| High-Throughput Sequencing | ~0.1% | Y | Y |
1 ~1% for deletions. 2 we marked Y for yes and N for no. The word ‘recorded’ represents that this method can only detect the present variations which are recorded in datasets. The absence of ‘recorded’ means both of the recorded and novel variations can be detected.