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. 2018 Jan 24;23(2):209. doi: 10.3390/molecules23020209

Figure 4.

Figure 4

Quercetin inhibits cell migration and downregulates epithelial–mesenchymal transition (EMT)-related protein expression in AGS-cyr61 cells. (A) Wound-healing assay of AGS and AGS-cyr61 cells. After a confluent monolayer was formed, the cell monolayer was scratched with the sterile tip of a 200 μL micropipette. AGS cells were treated with a vehicle control (dimethyl sulfoxide [DMSO]), and AGS-cyr61 cells were treated with a vehicle control (DMSO) and quercetin (10 and 20 μM) for 48 h; (B) Percentage wound closure 48 h after DMSO and quercetin treatment, as in (A). Data are the means ± standard error for three independent experiments. * p < 0.05 compared with the control of AGS-cyr61 cells; (C) AGS-cyr61 cells were treated with quercetin for 24 h. Cell lysates were analyzed by western blotting for EMT-related proteins.