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. 2018 Jan 4;23(1):109. doi: 10.3390/molecules23010109

Figure 3.

Figure 3

The effects of compounds 13 on LPS-induced iNOS/COX-2 expression and I-κBα degradation in BV-2 microglia. (a) The effects of compounds 13 on LPS-stimulated iNOS and COX-2 protein expression. The protein levels were determined by Western blot analysis. The relative intensity of iNOS/COX-2 to β-actin bands was measured by densitometry. The values represent the means ± S.D. of three experiments. * p < 0.05; (b) The effects of compounds 13 on LPS-stimulated iNOS and COX-2 mRNA expression. The mRNA levels were examined by RT-PCR analysis. The relative intensity of iNOS/COX-2 to β-actin bands was measured by densitometry. The values represent the means ± S.D. of three experiments. * p < 0.05; (c) The effects of compounds 13 on LPS-induced I-κBα degradation and p65 level in nucleus of BV-2 microglia. Cells were treated with compounds 13 for 30 min prior to activation of LPS (0.1 μg/mL). After 15 min treatment of LPS, cytosolic I-κBα and nuclear p65 were analyzed by western blot. Images are the representative of three independent experiments that show similar results.