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. 2018 Apr 4;23(4):830. doi: 10.3390/molecules23040830

Figure 2.

Figure 2

Schematics of aptamer-oligonucleotides and aptamer-drug conjugates. (A) Aptamer-siRNA conjugate. A PSMA aptamer is covalently conjugated with Plk1 siRNA via a linker; (B) Aptamer stick sequence-siRNA conjugate. A BAFF-R aptamer is complexed with STAT3 siRNA via a pair of complementary stick sequences; (C) Aptamer stick sequence-miRNA conjugate. The stick sequence (17-mer) of the GL21.T aptamer and extended stick sequence of anti-miRNA-222 at the 3′-end are fully annealed; (D) Aptamer stick sequence-dual anti-miRNA conjugate. Anti-miRNA-10b and anti-miRNA-222 are conjugated with the GL21.T aptamer; (E) Bivalent aptamer-shRNA conjugate. Two CD40 aptamers are conjugated with SMG1 shRNA; (F) Three-way junction-aptamer-anti-miRNA conjugate. The trifunctional aptamer consists of four DNA strands containing EGFR aptamer, anti-miRNA-21, and Alexa 647 dye; (G) Aptamer-drug conjugate. An AS1411 aptamer is directly conjugated with Dox by incubation with formaldehyde; (H) Aptamer-linker-drug conjugate. An Sgc8c aptamer and Dox or PSMA aptamer and gelonin are conjugated via a linker; (I) Aptamer-multiple drug conjugate. Sgc8 aptamer is conjugated with modified phosphoramidite containing a photocleavable linker and an anti-cancer drug; (J) Drug-intercalating aptamer. Dox is loaded into double-stranded regions of the PSMA aptamer or MUC1 aptamer; (K) Aptamer-cargo conjugate. A CD38 aptamer conjugated with the CG-rich sequence of “CG cargo” carries Dox; (L) Aptamer-dual cargo conjugate. A G-quadruplex DNA aptamer is conjugated with two double-stranded DNAs carrying Dox by a linker; (M) Polyvalent aptamer-drug conjugate. Multiple aptamers specific to leukemia cells are synthesized by rolling circle amplification and Dox is loaded between the aptamer units; (N) Aptamer-tethered DNA nanotrains. An Sgc8c aptamer and two short DNA strands were assembled to form “nanotrains”.