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Flaccidoxide-13-acetate regulated the expression levels of mTOR signaling pathway proteins. Western blotting analysis demonstrated the changes in expressions levels of FAK, PI3K, phosphorylated-PI3K, AKT, phosphorylated-AKT, mTOR and phosphorylated-mTOR in T24 and RT4 cells treated with flaccidoxide-13-acetate. C: cells treated with DMSO vehicle only. β-actin was used as the protein loading control.
