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. 2018 Feb 27;23(3):526. doi: 10.3390/molecules23030526

Figure 1.

Figure 1

rv3623/lpqG gene presence and transcription. (A) Amplification of hsp65 gene from gDNA isolated from: 1. Mtb H37Rv; 2. Mtb H37Ra; 3. M. bovis; 4. M. bovis BCG; 5. M. smegmatis; 6. Positive PCR control; NC: Negative PCR control. MWM: 50 bp molecular weight marker; (B) Amplification of rv3623 gene from gDNA isolated from: 1. Mtb H37Rv; 2. Mtb H37Ra; 3. M. bovis; 4. M. bovis BCG; 5. M. smegmatis; 6. Positive PCR control; NC: Negative PCR control; (C) hsp65 gene amplification from cDNA of: 1. Mtb H37Rvplus synthesis; 2. Mtb H37Rvminus synthesis; 3. Mtb H37Raplus synthesis; 4. Mtb H37Raminus synthesis; 5. M. bovis plus synthesis; 6. M. bovis minus synthesis; 7. M. bovis BCGplus synthesis; 8. M. bovis BCGminus synthesis; 9. M. smegmatis plus synthesis; 10. M. smegmatis minus synthesis; 11. Positive PCR control (Mtb H37RvgDNA); NC: Negative PCR control; MWM: 50 bp molecular weight marker; (D) rv3623 gene amplification from cDNA of: 1. Mtb H37Rv; 2. Mtb H37Ra; 3. M. bovis; 4. M. bovis BCG; 5. M. smegmatis; 6. Positive PCR control; NC: Negative PCR control. MWM: molecular weight marker.