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. 2018 Feb 27;23(3):526. doi: 10.3390/molecules23030526

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Western blot recognition of Rv3623 in M. tuberculosis. Lane 1: pre-immune sera. Lane 2: post-third immunisation of the 16660/16665/BSA mixture against lysed Mtb H37Rv. Lanes 3 and 7: recognition of lysed Mtb H37Rv by hyper-immune sera control (obtained by inoculating mice with Mtb H37Rv sonicate). Identification of membrane (lane 4), wall (lane 5) and cytosol proteins (lane 6) lysed from H37Rv by sera from mice immunized with the 16660/16665/BSA mixture (25 µg antigen was added to each lane).