Figure 3.
Binding ability of SP1 to c-KIT (A); VEGF (B); BCL-2 (C); HRAS1 (D); and HRAS2 (E) G4 DNAs in the presence of K+ or Li+. Binding of GST-SP1 to G4 DNAs immobilized on the well was detected with an HRP-conjugated anti-GST antibody. As a negative control, a well without immobilized DNA was utilized. Bar plots mean values ± SD (N = 3). Statistical analyses were performed using two-way ANOVA with Tukey’s multiple comparisons post-hoc tests. The significance of differences of observed values from the Li+-treated group are indicated as follows: * p < 0.05; ** p < 0.001; *** p < 0.0005; **** p < 0.0001.