Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 24;23(2):211. doi: 10.3390/molecules23020211

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Dependence of enzymatic reaction rates on para Hammett constant, for StNfsB (green) and MsPnbA (purple) [77]. (a) The second-order rate constant (k_cat/K_M) for each substituted substrate was divided by the value obtained for (unsubstituted) nitrobenzene before taking the log to get log[(k_cat/K_M)/(k_cat/K_M)₀], which was plotted against the Hammett para substituent constant σ. Log[(k_cat/K_M)/(k_cat/K_M)₀] plots yielded ρ values of 3.1 ± 0.2 (StNfsB, R² = 0.86) and 2.9 ± 0.2 (MsPnbA, R² = 0.83); (b) the first-order rate constants are plotted without normalization, yielding for StNfsB a slope of 2.4 ± 0.4 and an intercept of 0.9 ± 0.3 (R² = 0.88), and for MsPnbA a slope of 1.0 ± 0.1 and intercept of 0.5 ± 0.08 (R² = 0.92). See Methods for the equation and also Supplemental Table S2 and Figure S4 for compounds, and details.