Table 6.

The dilution integrity of the fourteen alkaloids in ICR mice plasma (mean ± SD, n = 5).

Alkaloids	Spiked Conc. (ng/mL)	Dilution Factor	Measured Conc. (ng/mL)	Accuracy (%, RE)	Precision (%, RSD)
Corydaline (1)	369.45	10	33.07 ± 1.96	−10.5	5.9
		20	18.16 ± 0.80	−1.7	4.4
Tetrahydroberberine (2)	339.38	10	36.05 ± 1.27	6.2	3.5
		20	18.27 ± 1.02	7.7	5.6
Tetrahydropalmatine (3)	355.43	10	38.57 ± 2.32	8.5	6.0
		20	17.92 ± 0.15	0.8	0.8
Tetrahydrocolumbamine (4)	341.4	10	34.74 ± 0.66	0.0	3.7
		20	17.57 ± 0.65	−1.1	1.9
Tetrahydrocoptisine (5)	323.34	10	31.37 ± 1.25	−3.0	3.1
		20	15.67 ± 0.49	−3.0	4.0
Columbamine (6)	338.38	10	34.15 ± 2.06	0.9	6.0
		20	16.95 ± 0.49	0.2	2.9
Palmatine (7)	352.4	10	36.54 ± 1.73	3.7	4.7
		20	19.63 ± 0.17	11.4	0.8
Berberine (8)	336.36	10	31.44 ± 1.90	−6.5	6.0
		20	14.80 ± 0.70	−12.0	4.8
Epiberberine (9)	336.36	10	34.05 ± 2.08	1.2	6.1
		20	16.04 ± 0.65	−4.6	4.0
Coptisine (10)	1601.6	10	165.3 ± 6.02	3.2	3.6
		20	83.42 ± 1.79	4.2	2.2
Jatrorrhizine (11)	338.38	10	34.33 ± 0.64	0.4	2.6
		20	16.99 ± 0.45	1.4	1.9
Dehydrocorydaline (12)	366.43	10	34.71 ± 0.61	−4.0	8.4
		20	17.59 ± 1.48	−5.3	1.7
Oxoglaucine (13)	351.35	10	36.73 ± 1.92	4.5	5.2
		20	17.53 ± 0.84	−0.2	4.8
Protopine (14)	353.37	10	36.66 ± 2.20	3.8	6.0
		20	17.39 ± 0.81	−1.6	4.7