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. 2018 Apr 17;23(4):926. doi: 10.3390/molecules23040926

Figure 1.

Figure 1

Cell viability (A) and nitric oxide (NO) production (B) of total extract and fractions from Selaginella tamariscina. RAW 264.7 cells were cultured in the presence of total extract and each fraction for 1 h and stimulated with lipopolysaccharide (LPS) (1 μg/mL) for 24 h under serum-free conditions. Cell viabilities and NO production were determined using cell counting kit (CCK)-8 assay and Griess reagent, respectively. Nitrite concentrations of nontreated and LPS-treated controls were 4.63 ± 1.48 μM and 30.35 ± 0.12 μM, respectively. Each determination was made in triplicate. Data are presented as means ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. LPS-treated group.